Part:BBa_K3019011:Design

α-factor pre-pro signal GLCI

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 549
  Illegal AgeI site found at 1630
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

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Source

Alfa factor pre-pro signal sequence was taken from yeast genomic DNA. glcI gene is from Arthrobacter sp. YCWD3. The sequence of the part was obtained from NCBI database (https://www.ncbi.nlm.nih.gov/nuccore/D23668.1?report=fasta) and codon optimised for S. Cerevisiae